The efficiency and result reliability of Western Blot (WB) experiments largely depend on the understanding of antibody characteristics, correct antibody selection, and optimization of the experimental workflow. To address common issues encountered during WB experiments, Dr. Joanna, a technical expert from Agrisera, will deliver a series of lectures on comprehensive WB workflow optimization. This lecture series consists of two online live sessions. The first session will be broadcast live at 4:00 PM on June 18th, titled "From Antibody Selection to Comprehensive WB Workflow Optimization Strategy". It will conduct an in-depth analysis of the key steps in Western Blot technology and provide you with professional optimization solutions. We welcome you to register and reserve your spot in advance.

Lecture Topic: From Antibody Selection to Comprehensive Western Blot (WB) Workflow Optimization Strategy - Part I

Time: Wednesday, June 18, 2025, 16:00-17:00

Speaker: Dr. Joanna

Technical Support Manager at Agrisera, with over 20 years of experience in antibody validation

Main Content:

How to select the appropriate antibody?

· Three key factors for antibody selection: experimental application (WB, IHC, IF, etc.), target species, and sample type.

· Interpretation of core information in antibody datasheets: evaluation of specificity validation data, recommended applications, and reference literature.

· Learn how to avoid common issues such as cross-reactivity.

How do antibodies recognize target proteins?

· The consistency requirement for the binding sequence between antibodies and antigens.

· Detailed background information of target proteins (e.g., protein properties, expression levels, subcellular localization).

How to efficiently extract protein samples?

· Key factors affecting protein extraction efficiency.

· Questions to clarify before protein extraction (experimental objectives, tissue/sample type).

· Troubleshooting for common problems in protein extraction.

Western Blot (WB) Operation Workflow and Optimization Strategies

· Detailed explanation of core WB steps: SDS-PAGE sample loading, electrophoresis, membrane transfer, blocking, primary/secondary antibody incubation, washing, and detection.

· Optimization strategies for major experimental steps.

Registration & Reservation:

Please scan the QR code below to register. Seats are limited and available on a first-come, first-served basis.

Attendee Benefits:

· Agrisera Course Certificate

· PDF Version of the Lecture Script with Practical Links Included

· Lecture Replay Video

We look forward to interacting with you online at 16:00 (GMT+8) on Wednesday, June 18, 2025.

Agrisera AB (Sweden), represented by XMJ (Beijing) Technology Co., Ltd., has been dedicated to developing high-quality plant monoclonal and polyclonal antibodies since its establishment in 1980. With its outstanding R&D capabilities and production technology, it holds a leading position in the global plant antibody field.

Its product line covers a wide range of species. The Agrisera product catalog includes antibodies for numerous model plants and important cash crops—such as Arabidopsis thaliana, rice, wheat, corn, and tomato—spanning over 30 plant species. This extensive antibody library meets the diverse needs of various plant science research projects.

Moreover, Agrisera’s products are supported by a large number of published literatures, which ensures the accuracy and reliability of research results.

As the first-tier distributor of Agrisera, XMJ (Beijing) Technology Co., Ltd. provides customers with professional technical support, excellent after-sales service, and high-quality plant antibodies. If you are interested in Agrisera products, please feel free to send email to info@xmjsci.com or visit our official website www.lxhmstone.com for more information.